Human normal keratinocyte prepared from human foreskin that was immortalized by the infection of the pSV40 ori-(minus) DNA.
Biosafety Level:
1
Morphology:
epithelial-like
Medium:
MCDB153 medium (0.03 mM Ca2+) with 5 ug/ml insulin, 0.5 ug/ml hydrocortisone, 10 ug/ml transferrin, 0.1 mM phosphorylethanolamine, 0.1 mM ethanolamine, 10 ng/ml EGF and 40-70 ug/ml bovine pituitary ex
