Cella Single Cell Sequencing

Single Cell Sequencing

Single-cell sequencing is a technology used to isolate and examine nucleic acid sequences—whole genomes or transcriptomes-of individual cells. This method allows for multi-omics studies in cellular interactions and analysis of evolving cell populations. Single-cell sequencing provides a high-quality genomic picture of an individual cell, rather than a broad genomic snapshot from bulk DNA or RNA sequencing of tissue or sample. Single-cell sequencing is important in gene regulation studies and analyzing differences in gene expression between cells. Researchers can greatly benefit from single-cell sequencing in tumor studies and oncology research.

To perform single-cell RNA sequencing, you need to perform the following 5 steps:

Generate a single-cell suspension. To isolate the individual cells from each other in the next step, you need to create a single-cell suspension from your sample.

Isolate single cells. Because we want to barcode the cells individually in the next step, we need to isolate them from each other. Common ways to do this isolation is by FACS sorting or running the cells through a microfluidics system.

Barcoding and amplification. Once cells are isolated, we can label them with a barcode (a short sequence) to distinguish them during the data analysis. We then amplify the RNA to make sure we have sufficient quantities for the library preparation.

NGS library preparation and sequencing. We barcode the samples with a sample-specific barcode and pool the amplified RNA into a sequencing library.

Data analysis. For the analysis of the data, we map the raw sequencing data to the reference transcriptome. After this step, we can analyze the data using specialized data analysis pipelines.

By combining the 10x Genomics Chromium system and Illumina NGS platform, Cellalabs offers high-quality end-to-end single cell sequencing solutions and services, including Single Cell Gene Expression, Single Cell Immune Profiling, and Single Cell ATAC, etc.

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